быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
RAB45 Rabbit mAb (A21163)
- Описание
- Характеристики
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Overview
Product name RAB45 Rabbit mAb Catalog No. A21163 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3022 Background
This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene.Immunogen information
Immunogen Recombinant protein of Human RAB45. Sequence Email for sequence Gene ID Swiss Prot Synonyms TSG; RAB45 Calculated MW 83kDa Observed MW 83kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples SK-BR-3, T47D, LNCaP, K-562, Mouse stomach Cellular location Cytoplasm, perinuclear region 
Western blot analysis of various lysates, using RAB45 antibody (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of various lysates, using RAB45 antibody (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of Human RAB45. Isotype IgG