быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Siglec-8 Rabbit mAb (A21172)
- Описание
- Характеристики
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Overview
Product name Siglec-8 Rabbit mAb Catalog No. A21172 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52425 Background
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8 (NP_055257.2). Sequence MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA Gene ID Swiss Prot Synonyms SAF2; SIGLEC-8; SIGLEC8L; SIGLEC8 Calculated MW 54kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB FC Recommended dilution - WB 1:2000 - 1:10000
- FC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Flow Cytometry Positive samples 293T Cellular location plasma membrane 
Western blot analysis of extracts of normal 293T cells and 293T transfected with Siglec-8 protein, using Siglec-8 antibody (A21172) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were surface-stained with Siglec-8 Rabbit mAb(A21172, 10 μg/mL, green line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
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Key applications Western blotting, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8 (NP_055257.2). Isotype IgG