быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Galectin 1/LGALS1 Rabbit mAb (A21218)
- Описание
- Характеристики
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Overview
Product name Galectin 1/LGALS1 Rabbit mAb Catalog No. A21218 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53156 Background
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human Galectin 1/LGALS1 (NP_002296.1). Sequence MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Gene ID Swiss Prot Synonyms GBP; GAL1 Calculated MW 15kDa Observed MW 14kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples A549, U-2 OS, Mouse thymus, Mouse testis, Rat heart, Rat lung Cellular location Secreted, Extracellular matrix, Extracellular space 
Western blot analysis of extracts of various cell lines, using Galectin 1/LGALS1 antibody (A21218) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Galectin 1/LGALS1 Rabbit mAb (A21218) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using Galectin 1/LGALS1 Rabbit mAb (A21218) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Galectin 1/LGALS1 Rabbit mAb (A21218) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human Galectin 1/LGALS1 (NP_002296.1). Isotype IgG