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Размер
- 100 μL
- 200 μL
- 50 μL
Human IgG1 (Fc) Rabbit mAb (A21233)
- Описание
- Характеристики
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Overview
Product name Human IgG1 (Fc) Rabbit mAb Catalog No. A21233 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53385 Background
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to act upstream of or within several processes, including immunoglobulin mediated immune response; positive regulation of hypersensitivity; and positive regulation of phagocytosis. Located in extracellular space.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG1 (Fc) (P01857). Sequence PKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Gene ID Swiss Prot Synonyms Calculated MW 44kDa Observed MW 50kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC FC Recommended dilution - WB 1:2000 - 1:4000
- IHC-P 1:2000 - 1:6000
- FC 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Flow Cytometry Positive samples human plasma Cellular location 
Western blot analysis of extracts of human plasma, using Human IgG1 (Fc) antibody (A21233) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using Human IgG1 (Fc) Rabbit mAb (A21233) at dilution of 1:5000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of human tonsil using Human IgG1 (Fc) Rabbit mAb (A21233, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
Flow cytometry:1X10^6 293F cells (negative control, left) and Daudi cells (right) were intracellularly-stained with Human IgG1 (Fc) Rabbit mAb(A21233, 10 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were intracellularly-stained with Human IgG1 (Fc) Rabbit mAb(A21233, 10 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG1 (Fc) (P01857). KO Validated Validated Isotype IgG