быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263)
- Описание
- Характеристики
-
Overview
Product name Interferon alpha 2 (IFN-α2） Rabbit mAb Catalog No. A21263 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53291 Background
This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 24-188 of human Interferon alpha 2 (IFN-α2） (NP_000596.2). Sequence CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE Gene ID Swiss Prot Synonyms IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2 Calculated MW 22kDa Observed MW 20kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:2000 - 1:6000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T-Interferon alpha 2 (IFN-α2) Cellular location Extracellular region, Extracellular space 
Western blot analysis of 293T, using Interferon alpha 2 (IFN-α2） antibody (A21263) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse thymus using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using Interferon alpha 2 (IFN-α2） Rabbit mAb (A21263) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 7.2 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 24-188 of human Interferon alpha 2 (IFN-α2） (NP_000596.2). Isotype IgG