быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Human IgG (Fc) Rabbit mAb (A21291)
- Описание
- Характеристики
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Overview
Product name Human IgG (Fc) Rabbit mAb Catalog No. A21291 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53376 Background
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to act upstream of or within several processes, including immunoglobulin mediated immune response; positive regulation of hypersensitivity; and positive regulation of phagocytosis. Located in extracellular space.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG (Fc) mAb (P01857). Sequence PKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Gene ID Swiss Prot Synonyms Calculated MW 52kDa Observed MW 50-55kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:20000
- IHC-P 1:100 - 1:500
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Human plasma Cellular location 
Western blot analysis of extracts from control Human plasma, using HumanIgG(Fc) antibody (A21291) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Human IgG (Fc) Rabbit mAb (A21291) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of human tonsil using Human IgG (Fc) Rabbit mAb (A21291, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human Human IgG (Fc) mAb (P01857). Isotype IgG