быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
LDHA Rabbit mAb (A21893)
- Описание
- Характеристики
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Overview
Product name LDHA Rabbit mAb Catalog No. A21893 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0509 Background
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.Immunogen information
Immunogen Recombinant protein of human LDHA. Sequence Email for sequence Gene ID Swiss Prot Synonyms LDHM; GSD11; PIG19; HEL-S-133P Calculated MW 37kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293T, MCF7, Mouse liver, Rat lung, Rat kidney Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using LDHA Rabbit mAb (A21893) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human LDHA. Isotype IgG