быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TET3 Rabbit mAb (A21930)
- Описание
- Характеристики
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Overview
Product name TET3 Rabbit mAb Catalog No. A21930 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52663 Background
Enables methylcytosine dioxygenase activity. Involved in DNA demethylation of male pronucleus. Acts upstream of or within DNA demethylation. Located in cytoplasm; female pronucleus; and male pronucleus. Is expressed in several structures, including central nervous system; early conceptus; future brain; and genitourinary system. Orthologous to human TET3 (tet methylcytosine dioxygenase 3).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of mouse TET3 (Q8BG87). Sequence MSQFQVPLAVQPDLSGLYDFPQGQVMVGGFQGPGLPMAGSETQLRGGGDGRKKRKRCGTCDPCRRLENCGSCTSCTNRRTHQICKLRKCEVLKKKAGLLKEVEINAREGTGPWAQGATVKTGSELSPVDGPVPGQMDSGPVYHGDSRQLSTSGAPVNGAREPAGPGLLGAAGPWRVDQKPDWEAASGPTHAARLEDAHDLVAFSAVAEAVSSYGALSTRLYETFNREMSREAGSNGRGPRPESCSEGSEDLDTLQTALALARHGMKPPNCTCDGPECPDFLEWLE Gene ID Swiss Prot Synonyms B430006D22Rik; D230004J03Rik Calculated MW 195kDa Observed MW 230kDa Applications
Reactivity Mouse Tested applications WB Recommended dilution - WB 1:2000 - 1:20000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Neuro-2a Cellular location cytoplasm, female pronucleus, male pronucleus, nucleoplasm, nucleus 
Western blot analysis of extracts from Neuro-2a cells, using TET3 Rabbit mAb antibody (A21930) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of mouse TET3 (Q8BG87). Isotype IgG