быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
L3MBTL3 Rabbit mAb (A21932)
- Описание
- Характеристики
-
Overview
Product name L3MBTL3 Rabbit mAb Catalog No. A21932 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54019 Background
This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human L3MBTL3 (NP_115814.1). Sequence MTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDENEMENVKKATATTTWMVPTAQEAPTSPPSSRPVFPPAYWTSPPGCPTVFSEKTGMPFRLKDPVKVEGLQFCENCCQYGNVDECLSGGNYCSQNCARHIKDKDQKEERDVEEDNEEEDPKCSRKKKPKLSLKADTKEDGEERDDEMENKQDVRILRGSQRARRKRRGDSAVLKQGLPPKGKKAWCWASYLEEEKAVAVPAKLFKEHQ Gene ID Swiss Prot Synonyms MBT1; MBT-1 Calculated MW 88kDa Observed MW 100kDa Applications
Reactivity Human Tested applications WB IP Recommended dilution - WB 1:2000 - 1:20000
- IP 150 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples 293F Cellular location Nucleus 
Western blot analysis of 293F, using L3MBTL3 antibody (A21932) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunoprecipitation analysis of 300 μg extracts from 293F cells 3 μg L3MBTL3 Rabbit mAb (A21932 1:100).
Western blot was performed from the immunoprecipitate using L3MBTL3 Rabbit mAb (A21932) at a dilution of 1:10000.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:5000 dilution. -
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human L3MBTL3 (NP_115814.1). Isotype IgG