быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Mesothelin Rabbit mAb (A21982)
- Описание
- Характеристики
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Overview
Product name Mesothelin Rabbit mAb Catalog No. A21982 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54390 Background
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2). Sequence EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS Gene ID Swiss Prot Synonyms MPF; SMRP Calculated MW 69kDa Observed MW 45kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples OVCAR3 Cellular location Cell membrane, GPI-anchor, Golgi apparatus, Lipid-anchor, Secreted 
Western blot analysis of extracts of OVCAR3 cell, using Mesothelin antibody (A21982) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.1s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Mesothelin Rabbit mAb (A21982) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2). Isotype IgG