быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CXCL10/IP-10 Rabbit mAb (A21986)
- Описание
- Характеристики
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Overview
Product name CXCL10/IP-10 Rabbit mAb Catalog No. A21986 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC5116-05 Background
This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. This gene may also be a key regulator of the 'cytokine storm' immune response to SARS-CoV-2 infection.Immunogen information
Immunogen Recombinant protein of human CXCL10/IP-10. Sequence Email for sequence Gene ID Swiss Prot Synonyms C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10 Calculated MW 11kDa Observed MW 11kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:2000 - 1:20000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples THP-1（THP-1 cells were treated by LPS for 6 hours, Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation.） Cellular location Secreted 
Western blot analysis of various lysates, using CXCL10/IP-10 antibody (A21986) at 1:20000 dilution.THP-1 cells were treated by LPS for 6 hours, Brefeldin A for 3 hours and IFNγ (50ng/ml) for 8 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human CXCL10/IP-10. Isotype IgG