быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Calretinin Rabbit mAb (A22010)
- Описание
- Характеристики
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Overview
Product name Calretinin Rabbit mAb Catalog No. A22010 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53573 Background
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human Calretinin (NP_001731.2). Sequence MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM Gene ID Swiss Prot Synonyms CR; CAL2; CAB29 Calculated MW 32kDa Observed MW 29kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:10000 - 1:130000
- IHC-P 1:50 - 1:200
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeL, Mouse brain, Rat brain Cellular location cytosol, dendrite, neuron projection, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton 
Western blot analysis of extracts of various cell lines, using Calretinin antibody (A22010) at1:110000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human appendix using Calretinin Rabbit mAb (A22010) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of 293F(Negative sample) cells using Calretinin Rabbit mAb (A22010) at dilution of 1:500(40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using Calretinin Rabbit mAb (A22010) at dilution of 1:500(40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat brain using Calretinin Rabbit mAb (A22010) at dilution of 1:500(40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human Calretinin (NP_001731.2). KO Validated Validated Isotype IgG