быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Progesterone Receptor Rabbit mAb (A22061)
- Описание
- Характеристики
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Overview
Product name Progesterone Receptor Rabbit mAb Catalog No. A22061 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC5121-01 Background
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap.Immunogen information
Immunogen Recombinant protein of human Progesterone Receptor. Sequence Email for sequence Gene ID Swiss Prot Synonyms PR; NR3C3 Calculated MW 99kDa Observed MW 90kDa/118kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples T-47D, Mouse ovary, Mouse pituitary, Rat uterus, Rat pituitary Cellular location Cytoplasm, Cytoplasm, Mitochondrion outer membrane, Nucleus 
Western blot analysis of extracts of T-47D cells, using Progesterone Receptor antibody (A22061) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 4s.
Western blot analysis of extracts of various cell lines, using Progesterone Receptor antibody (A22061) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Progesterone Receptor. Isotype IgG