быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
StAR Rabbit mAb (A22166)
- Описание
- Характеристики
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Overview
Product name StAR Rabbit mAb Catalog No. A22166 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC55812 Background
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human StAR (NP_000340.2). Sequence SQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLA Gene ID Swiss Prot Synonyms STARD1 Calculated MW 32kDa Observed MW 28kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples K-562, Mouse testis, Rat testis Cellular location Mitochondrion 
Western blot analysis of various lysates, using StAR antibody (A22166) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of exactras from K-562 cells, using StAR antibody (A22166) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of Human adrenal gland using StAR Rabbit mAb (A22166) at dilution of 1:100(40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse adrenal gland using StAR Rabbit mAb (A22166) at dilution of 1:100(40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat adrenal gland using StAR Rabbit mAb (A22166) at dilution of 1:100(40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human StAR (NP_000340.2). Isotype IgG