быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD146/MCAM Rabbit mAb (A22195)
- Описание
- Характеристики
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Overview
Product name CD146/MCAM Rabbit mAb Catalog No. A22195 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53805 Background
Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 26-350 of human CD146/MCAM (NP_006491.2). Sequence GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP Gene ID Swiss Prot Synonyms CD146; MUC18; HEMCAM; METCAM; MelCAM Calculated MW 72kDa Observed MW 120kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:2000 - 1:10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Membrane, Single-pass type I membrane protein 
Western blot analysis of HeLa, using CD146/MCAM antibody (A22195) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:2000000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 26-350 of human CD146/MCAM (NP_006491.2). Isotype IgG