быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
DGKA Rabbit mAb (A22202)
- Описание
- Характеристики
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Overview
Product name DGKA Rabbit mAb Catalog No. A22202 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC55770 Background
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_001336.2). Sequence MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTL Gene ID Swiss Prot Synonyms DAGK; DAGK1; DGK-alpha Calculated MW 83kDa Observed MW 80kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:2000 - 1:20000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Jurkat Cellular location cytosol, plasma membrane 
Western blot analysis of Jurkat, using DGKA antibody (A22202) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using DGKA Rabbit mAb (A22202) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_001336.2). Isotype IgG