быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MRP1/ABCC1 Rabbit mAb (A2223)
- Описание
- Характеристики
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Overview
Product name MRP1/ABCC1 Rabbit mAb Catalog No. A2223 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52207 Background
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 210-304 of human MRP1/ABCC1 (NP_004987.2). Sequence ESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKS Gene ID Swiss Prot Synonyms MRP; ABCC; GS-X; MRP1; ABC29; DFNA77 Calculated MW 172kDa Observed MW 170-220kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples A549, Mouse lung, Rat skeletal muscle, Rat heart Cellular location apical plasma membrane, basal plasma membrane, basolateral plasma membrane, extracellular exosome, lateral plasma membrane, plasma membrane 
Western blot analysis of extracts of A-549 cells, using MRP1/ABCC1 antibody (A2223) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1S.
Western blot analysis of extracts of various cell lines, using MRP1/ABCC1 antibody (A2223) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon using MRP1/ABCC1 antibody (A2223) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MRP1/ABCC1 antibody (A2223) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 210-304 of human MRP1/ABCC1 (NP_004987.2). Isotype IgG