быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
IP6K1 Rabbit mAb (A22272)
- Описание
- Характеристики
-
Overview
Product name IP6K1 Rabbit mAb Catalog No. A22272 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC56744 Background
This gene encodes a member of the inositol phosphokinase family. The encoded protein may be responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1, 3, 4, 5, 6-pentakisphosphate (InsP5) to PP-InsP4. Alternatively spliced transcript variants have been described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IP6K1 (NP_695005.1). Sequence MCVCQTMEVGQYGKNASRAGDRGVLLEPFIHQVGGHSSMMRYDDHTVCKPLISREQRFYESLPPEMKEFTPEYKGVVSVCFEGDSDGYINLVAYPYVESETVEQDDTTEREQPRRKHSRRSLHRSGSGSDHKEEKASLSLETSESSQEAKSPKVELHSHSEVPFQMLDGNSGLSSEKISHNPWSLRCHKQQLSRMRSESKDRKLYKFLLLENVVHHFKYP Gene ID Swiss Prot Synonyms PiUS; IHPK1 Calculated MW 50kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, HepG2, Mouse brain, Mouse lung, Rat brain Cellular location Cytoplasm, Nucleus 
Western blot analysis of various lysates, using IP6K1 antibody (A22272) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using IP6K1 Rabbit mAb (A22272) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IP6K1 (NP_695005.1). Isotype IgG