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Размер
- 100 μL
- 200 μL
- 50 μL
Axl Rabbit mAb (A22388)
- Описание
- Характеристики
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Overview
Product name Axl Rabbit mAb Catalog No. A22388 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53013 Background
The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 33-449 of human Axl (NP_068713.2). Sequence EESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWP Gene ID Swiss Prot Synonyms ARK; UFO; JTK11; Tyro7 Calculated MW 98kDa Observed MW 138kDa Applications
Reactivity Human Tested applications WB FC Recommended dilution - WB 1:1000 - 1:5000
- FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Flow Cytometry Positive samples HeLa Cellular location Cell membrane, Single-pass type I membrane protein 
Western blot analysis of various lysates, using Axl antibody (A22388) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and U-251MG cells (right) were surface-stained with Axl Rabbit mAb (A20548, 10 μg/mL orange line) or Rabbit IgG isotype control (AC042, 2.5 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HeLa cells (right) were surface-stained with Axl Rabbit mAb (A20548, 10 μg/mL orange line) or Rabbit IgG isotype control (AC042, 2.5 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
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Key applications Western blotting, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 33-449 of human Axl (NP_068713.2). Isotype IgG