быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ITK/EMT Rabbit mAb (A22617)
- Описание
- Характеристики
-
Overview
Product name ITK/EMT Rabbit mAb Catalog No. A22617 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58362 Background
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.Immunogen information
Immunogen Recombinant protein of human ITK/EMT. Sequence Email for sequence Gene ID Swiss Prot Synonyms EMT; LYK; LPFS1; PSCTK2 Calculated MW 72kDa Observed MW 75kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:2000 - 1:6000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat, Molt-4 Cellular location Cytoplasm 
Western blot analysis of various lysates, using ITK/EMT antibody (A22617) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human ITK/EMT. Isotype IgG