быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MTHFD2 Rabbit mAb (A22653)
- Описание
- Характеристики
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Overview
Product name MTHFD2 Rabbit mAb Catalog No. A22653 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58228 Background
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.Immunogen information
Immunogen Recombinant protein of human MTHFD2. Sequence Email for sequence Gene ID Swiss Prot Synonyms NMDMC Calculated MW 38kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:2000 - 1:8000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HT-29, HeLa, 293F, Mouse testis Cellular location Mitochondrion 
Western blot analysis of various lysates, using MTHFD2 antibody (A22653) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human MTHFD2. Isotype IgG