быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
FTCD Rabbit mAb (A22654)
- Описание
- Характеристики
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Overview
Product name FTCD Rabbit mAb Catalog No. A22654 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58229 Background
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.Immunogen information
Immunogen Recombinant protein of human FTCD. Sequence Email for sequence Gene ID Swiss Prot Synonyms LCHC1 Calculated MW 59kDa Observed MW 58kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HepG2, Mouse liver Cellular location Cytoplasm, Golgi apparatus, centriole, centrosome, cytoskeleton, microtubule organizing center 
Western blot analysis of various lysates, using FTCD antibody (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of Mouse liver, using FTCD antibody (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human liver using FTCD Rabbit mAb (A22654) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using FTCD Rabbit mAb (A22654) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human FTCD. Isotype IgG