быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
INTS11 Rabbit mAb (A22698)
- Описание
- Характеристики
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Overview
Product name INTS11 Rabbit mAb Catalog No. A22698 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58804 Background
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).Immunogen information
Immunogen Recombinant protein of human INTS11. Sequence Email for sequence Gene ID Swiss Prot Synonyms RC68; INT11; RC-68; CPSF3L; CPSF73L Calculated MW 68kDa Observed MW 68kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:2000 - 1:7000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293F, Mouse spleen, Rat testis Cellular location blood microparticle, cytoplasm, cytosol, nucleoplasm, nucleus 
Western blot analysis of various lysates, using INTS11 antibody (A22698) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human INTS11. Isotype IgG