быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD33 Rabbit mAb (A22704)
- Описание
- Характеристики
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Overview
Product name CD33 Rabbit mAb Catalog No. A22704 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57811 Background
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.Immunogen information
Immunogen Recombinant protein of human CD33. Sequence Email for sequence Gene ID Swiss Prot Synonyms p67; SIGLEC3; SIGLEC-3 Calculated MW 40kDa Observed MW 70-80kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:2000 - 1:8000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples U-937, THP-1 Cellular location Cell membrane, Single-pass type I membrane protein 
Western blot analysis of various lysates, using CD33 antibody (A22704) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human CD33. Isotype IgG