быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NFATC3 Rabbit mAb (A22710)
- Описание
- Характеристики
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Overview
Product name NFATC3 Rabbit mAb Catalog No. A22710 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58477 Background
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.Immunogen information
Immunogen Recombinant protein of human NFATC3. Sequence Email for sequence Gene ID Swiss Prot Synonyms NFAT4; NFATX; NF-AT4c; n339260 Calculated MW 116kDa Observed MW 150kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:2000 - 1:9000
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Jurkat Cellular location Cytoplasm, Nucleus 
Western blot analysis of Jurkat, using NFATC3 antibody (A22710) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of mouse thymus using NFATC3 Rabbit mAb (A22710) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat thymus using NFATC3 Rabbit mAb (A22710) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human NFATC3. Isotype IgG