быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TCAP Rabbit mAb (A22731)
- Описание
- Характеристики
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Overview
Product name TCAP Rabbit mAb Catalog No. A22731 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58539 Background
Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.Immunogen information
Immunogen Recombinant protein of human TCAP. Sequence Email for sequence Gene ID Swiss Prot Synonyms TELE; CMD1N; CMH25; T-cap; LGMD2G; LGMDR7; telethonin Calculated MW 19kDa Observed MW 19kDa/21kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:2000 - 1:6000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293F-TCAP-C, Mouse heart Cellular location Cytoplasm, myofibril, sarcomere 
Western blot analysis of Mouse heart, using TCAP antibody (A22731) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of 293F-TCAP-c, using TCAP antibody (A22731) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of mouse heart using TCAP Rabbit mAb (A22731) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using TCAP Rabbit mAb (A22731) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal imaging of Human skeletal muscle using TCAP Rabbit mAb (A22731, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human TCAP. Isotype IgG