быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SUMO2/3 Rabbit mAb (A22734)
- Описание
- Характеристики
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Overview
Product name SUMO2/3 Rabbit mAb Catalog No. A22734 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57112 Background
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Immunogen information
Immunogen Recombinant protein of human SUMO2/3. Sequence Email for sequence Gene ID Swiss Prot Synonyms HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2 Calculated MW 11kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293F, NIH/3T3, PC-12 Cellular location Nucleus, PML body 
Western blot analysis of 293F, using SUMO2/3 antibody (A22734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of various lysates, using SUMO2/3 antibody (A22734) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Confocal imaging of HeLa cells using SUMO2/3 Rabbit mAb (A22734, dilution 1:400)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human SUMO2/3. Isotype IgG