быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NGLY1 Rabbit mAb (A22761)
- Описание
- Характеристики
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Overview
Product name NGLY1 Rabbit mAb Catalog No. A22761 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59085 Background
This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2) Sequence MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLDKGINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEK Gene ID Swiss Prot Synonyms CDDG; PNG1; CDG1V; PNG-1; PNGase Calculated MW 74kDa Observed MW 76kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples K-562, HUH-7(Negative control), U266, Mouse lung, Rat lung Cellular location Cytoplasm 
Western blot analysis of various lysates, using NGLY1 antibody (A22761) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human colon using NGLY1 Rabbit mAb (A22761) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using NGLY1 Rabbit mAb (A22761) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using NGLY1 Rabbit mAb (A22761) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using NGLY1 Rabbit mAb (A22761) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using NGLY1 Rabbit mAb (A22761) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2) Isotype IgG