быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
S100A9 Rabbit PolymAb® (A22987)
- Описание
- Характеристики
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Overview
Product name S100A9 Rabbit PolymAb® Catalog No. A22987 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC55864_ARC55869 Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 ( NP_002956.1). Sequence Email for sequence Gene ID Swiss Prot Synonyms MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387; S100-A9 Calculated MW 13kDa Observed MW 15kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples U-937, Jurkat (Negative control) , Mouse spleen, Rat lung Cellular location Secreted, Cytoplasm, Cell membrane 
Western blot analysis of various lysates, using S100A9 Rabbit PolymAb® (A22987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.
Western blot analysis of various lysates, using S100A9RabbitPolymAb® (A22987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human cervix cancer using S100A9 Rabbit PolymAb® (A22987) at dilution of 1:5000/15000/45000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using S100A9 Rabbit PolymAb® (A22987) at dilution of 1:5000/15000/45000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human spleen using S100A9 Rabbit PolymAb® (A22987) at dilution of 1:5000/15000/45000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 (NP_002956.1). Isotype IgG