быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD141/Thrombomodulin Rabbit PolymAb® (A22989)
- Описание
- Характеристики
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Overview
Product name CD141/Thrombomodulin Rabbit PolymAb® Catalog No. A22989 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52599_ARC52600 Background
The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia.Immunogen information
Immunogen Recombinant protein of human CD141/Thrombomodulin. Sequence Email for sequence Gene ID Swiss Prot Synonyms TM; THRM; AHUS6; BDCA3; CD141; BDCA-3; THPH12 Calculated MW 60kDa Observed MW 90kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples A431 cells Cellular location Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein 
Western blot analysis of extracts of A-431, using CD141/Thrombomodulin Rabbit PolymAb® (A22989) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using CD141/Thrombomodulin Rabbit PolymAb® (A22989) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A431 using CD141/Thrombomodulin Rabbit PolymAb® (A22989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 using CD141/Thrombomodulin Rabbit PolymAb® (A22989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human CD141/Thrombomodulin. KO Validated Validated Isotype IgG