быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CBL Rabbit mAb (A22994)
- Описание
- Характеристики
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Overview
Product name CBL Rabbit mAb Catalog No. A22994 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59894 Background
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.Immunogen information
Immunogen Recombinant protein of human CBL. Sequence Email for sequence Gene ID Swiss Prot Synonyms CBL2; NSLL; C-CBL; RNF55; FRA11B Calculated MW 100kDa Observed MW 110kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:100 - 1:500
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples Raji, Mouse thymus, Rat testis Cellular location Cell membrane, Cytoplasm 
Western blot analysis of various lysates, using CBL antibody (A22994) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human testis using CBL Rabbit mAb (A22994) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using CBL Rabbit mAb (A22994) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 600 μg extracts of Rat testis cells using 3 μg CBL antibody (A22994). Western blot was performed from the immunoprecipitate using CBL antibody (A22994) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human CBL. Isotype IgG