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Размер
- 100 μL
- 200 μL
- 50 μL
TFF1/pS2 Rabbit mAb (A22999)
- Описание
- Характеристики
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Overview
Product name TFF1/pS2 Rabbit mAb Catalog No. A22999 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59782 Background
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.Immunogen information
Immunogen A synthesized peptide derived from human TFF1/pS2. Sequence MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF Gene ID Swiss Prot Synonyms pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21 Calculated MW 9kDa Observed MW 13kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples MCF7, Mouse stomach, Rat stomach Cellular location extracellular region, extracellular space 
Western blot analysis of various lysates, using TFF1/pS2 antibody (A22999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Western blot analysis of various lysates, using TFF1/pS2 antibody (A22999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunofluorescence analysis of mouse spleen using TFF1/pS2 Rabbit mAb (A22999) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat stomach using TFF1/pS2 Rabbit mAb (A22999) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg TFF1/pS2 antibody (A22999). Western blot was performed from the immunoprecipitate using TFF1/pS2 antibody (A22999) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human TFF1/pS2. Isotype IgG