быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD8A Rabbit mAb (A23081)
- Описание
- Характеристики
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Overview
Product name CD8A Rabbit mAb Catalog No. A23081 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59319 Background
Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).Immunogen information
Immunogen Recombinant protein of mouse CD8A. Sequence Email for sequence Gene ID Swiss Prot Synonyms Ly-2; Ly-B; Ly-35; Lyt-2 Calculated MW 27kDa Observed MW 35-42kDa Applications
Reactivity Mouse Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse spleen, Mouse thymus, Mouse brain(Negative control) Cellular location 
Western blot analysis of various lysates, using CD8A antibody (A23081) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded mouse colon using CD8A Rabbit mAb (A23081) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD8A Rabbit mAb (A23081) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of mouse thymus using CD8A Rabbit mAb (A23081) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant protein of mouse CD8A. Isotype IgG