быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
GLI2 Rabbit mAb (A23094)
- Описание
- Характеристики
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Overview
Product name GLI2 Rabbit mAb Catalog No. A23094 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58878 Background
This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.Immunogen information
Immunogen A synthesized peptide derived from human GLI2. Sequence Email for sequence Gene ID Swiss Prot Synonyms CJS; HPE9; PHS2; THP1; THP2 Calculated MW Observed MW Applications
Reactivity Human, Mouse, Rat Tested applications IHC-P IF/ICC Recommended dilution - IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Immunohistochemistry Immunofluorescence Positive samples Cellular location Nucleus 
Immunohistochemistry analysis of paraffin-embedded human lung cancer using GLI2 Rabbit mAb (A23094) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using GLI2 Rabbit mAb (A23094) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using GLI2 Rabbit mAb (A23094) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 using GLI2 Rabbit mAb (A23094) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse ovary using GLI2 Rabbit mAb (A23094) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human GLI2. Isotype IgG