быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
COX1 Rabbit mAb (A23123)
- Описание
- Характеристики
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Overview
Product name COX1 Rabbit mAb Catalog No. A23123 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59830 Background
Enables cytochrome-c oxidase activity. Predicted to be involved in electron transport coupled proton transport; mitochondrial electron transport, cytochrome c to oxygen; and response to oxidative stress. Located in mitochondrial inner membrane. Part of mitochondrial respiratory chain complex IV. Is expressed in several structures, including brown fat; heart; liver; metanephros; and skeletal muscle. Orthologous to human MT-CO1 (mitochondrially encoded cytochrome c oxidase I).Immunogen information
Immunogen A synthesized peptide derived from human COX1. Sequence Email for sequence Gene ID Swiss Prot Synonyms CoxI; COX1 Calculated MW 57kDa Observed MW 37kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse heart, Rat heart Cellular location Mitochondrion inner membrane, Multi-pass membrane protein 
Western blot analysis of various lysates, using COX1 antibody (A23123) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human kidney using COX1 Rabbit mAb (A23123) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using COX1 Rabbit mAb (A23123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using COX1 Rabbit mAb (A23123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human COX1. Isotype IgG