быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Niemann-Pick type C2（NPC2） Rabbit mAb (A23128)
- Описание
- Характеристики
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Overview
Product name Niemann-Pick type C2（NPC2） Rabbit mAb Catalog No. A23128 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59098 Background
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.Immunogen information
Immunogen Recombinant protein of human Niemann-Pick type C2（NPC2）. Sequence Email for sequence Gene ID Swiss Prot Synonyms HE1; EDDM1 Calculated MW 17kDa Observed MW 16-21kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:500- 1:1000
- IF 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HepG2, 293F, NIH/3T3 Cellular location Endoplasmic reticulum, Lysosome, Secreted 
Western blot analysis of various lysates, using Niemann-Pick type C2（NPC2） antibody (A23128) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1：1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1：1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of human lung using Niemann-Pick type C2（NPC2） Rabbit mAb (A23128) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Niemann-Pick type C2（NPC2）. Isotype IgG