быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
FRS2 Rabbit PolymAb® (A23135)
- Описание
- Характеристики
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Overview
Product name FRS2 Rabbit PolymAb® Catalog No. A23135 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57138_ARC5714 Background
Enables fibroblast growth factor receptor binding activity and neurotrophin TRKA receptor binding activity. Involved in negative regulation of cardiac muscle cell differentiation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in adherens junction. Biomarker of renal cell carcinoma.Immunogen information
Immunogen Recombinant protein of human FRS2. Sequence Email for sequence Gene ID Swiss Prot Synonyms SNT; SNT1; FRS1A; FRS2A; SNT-1; FRS2alpha Calculated MW 57kDa Observed MW 80kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse kidney Cellular location Endomembrane system 
Western blot analysis of extracts from mouse kidney, using FRS2 Rabbit PolymAb® (A23135) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human kidney using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of PC-12 using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 using FRS2 Rabbit PolymAb® (A23135) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human FRS2. Isotype IgG