быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ATP6 Rabbit mAb (A23150)
- Описание
- Характеристики
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Overview
Product name ATP6 Rabbit mAb Catalog No. A23150 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59849 Background
Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6).Immunogen information
Immunogen A synthesized peptide derived from human ATP6. Sequence Email for sequence Gene ID Swiss Prot Synonyms ATP6 Calculated MW 25kDa Observed MW 20kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HepG2 Cellular location Mitochondrion inner membrane, Multi-pass membrane protein 
Western blot analysis of various lysates, using ATP6 antibody (A23150) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from human ATP6. Isotype IgG