быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Glutaminase (GLS) Rabbit mAb (A23189)
- Описание
- Характеристики
-
Overview
Product name Glutaminase (GLS) Rabbit mAb Catalog No. A23189 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59924 Background
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen A synthesized peptide derived from human Glutaminase (GLS). Sequence Email for sequence Gene ID Swiss Prot Synonyms GAC; GAM; KGA; GLS1; AAD20; DEE71; GDPAG; CASGID; EIEE71 Calculated MW 73kDa Observed MW 58kDa/65kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T, Mouse ovary Cellular location Cytoplasm, Mitochondrion, cytosol 
Western blot analysis of various lysates, using Glutaminase (GLS) Rabbit mAb (A23189) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 using Glutaminase (GLS) Rabbit mAb (A23189) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human Glutaminase (GLS). Isotype IgG