быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MAGED1/NRAGE Rabbit mAb (A23190)
- Описание
- Характеристики
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Overview
Product name MAGED1/NRAGE Rabbit mAb Catalog No. A23190 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59113 Background
This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human MAGED1/NRAGE. Sequence Email for sequence Gene ID Swiss Prot Synonyms NRAGE; DLXIN-1 Calculated MW 86kDa Observed MW 92-95kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples SH-SY5Y Cellular location Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein 
Western blot analysis of various lysates, using MAGED1/NRAGE Rabbit mAb (A23190) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat brain using MAGED1/NRAGE Rabbit mAb (A23190) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human MAGED1/NRAGE. Isotype IgG