быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD98/SLC3A2 Rabbit mAb (A23191)
- Описание
- Характеристики
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Overview
Product name CD98/SLC3A2 Rabbit mAb Catalog No. A23191 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59441 Background
This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.Immunogen information
Immunogen A synthesized peptide derived from human SLC3A2/CD98hc. Sequence Email for sequence Gene ID Swiss Prot Synonyms 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC Calculated MW 58kDa Observed MW 75-120kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HepG2, HeLa Cellular location anchoring junction, apical plasma membrane, basal plasma membrane, basolateral plasma membrane, extracellular exosome, nucleoplasm, plasma membrane 
Western blot analysis of various lysates, using SLC3A2/CD98hc Rabbit mAb (A23191) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of HepG2 cells using SLC3A2/CD98hc Rabbit mAb (A23191) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from human SLC3A2/CD98hc. Isotype IgG