быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD68 Rabbit mAb (A23205)
- Описание
- Характеристики
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Overview
Product name CD68 Rabbit mAb Catalog No. A23205 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC51152 Background
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.Immunogen information
Immunogen A synthesized peptide derived from human CD68. Sequence Email for sequence Gene ID Swiss Prot Synonyms GP110; LAMP4; SCARD1 Calculated MW 37kDa Observed MW 80-130kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P FC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:2000 - 1:9000
- FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Flow Cytometry Positive samples RAW264.7, THP-1, U-937 Cellular location Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein, Single-pass type I membrane protein 
Western blot analysis of various lysates, using CD68 Rabbit mAb (A23205) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon using CD68 Rabbit mAb (A23205) at dilution of 1:8000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using CD68 Rabbit mAb (A23205) at dilution of 1:8000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung using CD68 Rabbit mAb (A23205) at dilution of 1:8000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD68 Rabbit mAb (A23205) at dilution of 1:8000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthesized peptide derived from human CD68. KO Validated Validated Isotype IgG