быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
GALC Rabbit mAb (A23229)
- Описание
- Характеристики
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Overview
Product name GALC Rabbit mAb Catalog No. A23229 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58988 Background
This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Immunogen information
Immunogen Recombinant protein of human GALC. Sequence Email for sequence Gene ID Swiss Prot Synonyms GALC; galactocerebrosidase Calculated MW 77kDa Observed MW 80kDa/50kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples SH-SY5Y, A-549, Mouse Kidney, Mouse Testis, Rat Testis Cellular location Lysosome 
Western blot analysis of various lysates, using GALC Rabbit mAb (A23229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using GALC Rabbit mAb (A23229) at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human GALC. Isotype IgG