быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SETD2 Rabbit mAb (A23237)
- Описание
- Характеристики
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Overview
Product name SETD2 Rabbit mAb Catalog No. A23237 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58628 Background
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.Immunogen information
Immunogen Recombinant protein of human SETD2. Sequence Email for sequence Gene ID Swiss Prot Synonyms LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; MRD70; RAPAS; HBP231; HSPC069; p231HBP Calculated MW 288kDa Observed MW 310kDa Applications
Reactivity Mouse, Human, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Mouse spleen Cellular location Chromosome, Nucleus 
Western blot analysis of extracts from Mouse spleen, using SETD2 Rabbit mAb (A23237) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SETD2 Rabbit mAb (A23237) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer, using SETD2 Rabbit mAb (A23237) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver, using SETD2 Rabbit mAb (A23237) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney, using SETD2 Rabbit mAb (A23237) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Человек, Крыса Immunogen Recombinant protein of human SETD2. Isotype IgG