быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CHD1 Rabbit mAb (A23240)
- Описание
- Характеристики
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Overview
Product name CHD1 Rabbit mAb Catalog No. A23240 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59018 Background
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.Immunogen information
Immunogen Recombinant protein of human CHD1. Sequence Email for sequence Gene ID Swiss Prot Synonyms CHD-1; PILBOS Calculated MW 197kDa Observed MW 250kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples K-562 Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from K-562 cells, using CHD1 Rabbit mAb (A23240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse colon using CHD1 Rabbit mAb (A23240) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human CHD1. Isotype IgG