быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD102 (ICAM2) Rabbit mAb (A23245)
- Описание
- Характеристики
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Overview
Product name CD102 (ICAM2) Rabbit mAb Catalog No. A23245 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59377 Background
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 25-223 of human CD102 (ICAM2) (NP_000864.2). Sequence KVFEVHVRPK KLAVEPKGSL EVNCSTTCNQ PEVGGLETSL DKILLDEQAQ WKHYLVSNIS HDTVLQCHFT CSGKQESMNS NVSVYQPPRQ VILTLQPTLV AVGKSFTIEC RVPTVEPLDS LTLFLFRGNE TLHYETFGKA APAPQEATAT FNSTADREDG HRNFSCLAVL DLMSRGGNIF HKHSAPKMLE IYEPVSDSQ Gene ID Swiss Prot Synonyms CD102 Calculated MW 30KDa Observed MW 45kDa/55-80kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Jurkat, HUVEC Cellular location Membrane, Single-pass type I membrane protein 
Western blot analysis of various lysates, using CD102 (ICAM2) Rabbit mAb (A23245) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD102 (ICAM2) Rabbit mAb (A23245) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD102 (ICAM2) Rabbit mAb (A23245) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 25-223 of human CD102 (ICAM2) (NP_000864.2). Isotype IgG