быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
DARPP32 Rabbit mAb (A23282)
- Описание
- Характеристики
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Overview
Product name DARPP32 Rabbit mAb Catalog No. A23282 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59935 Background
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthesized peptide derived from human DARPP32. Sequence Email for sequence Gene ID Swiss Prot Synonyms DARPP32; DARPP-32 Calculated MW 23kDa Observed MW 32kDa/36kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:1000 - 1:5000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293F-PPP1R1B, Mouse brain Cellular location Cytoplasm 
Western blot analysis of 293F-PPP1R1B, using DARPP32 Rabbit mAb (A23282) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Western blot analysis of Mouse brain, using DARPP32 Rabbit mAb (A23282) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of 293F-PPP1R1B and 293F cells using DARPP32 Rabbit mAb (A23282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human DARPP32. Isotype IgG