быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
BLBP/FABP7 Rabbit mAb (A23286)
- Описание
- Характеристики
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Overview
Product name BLBP/FABP7 Rabbit mAb Catalog No. A23286 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60099 Background
The gene encodes a small, highly conserved cytoplasmic protein that bind long-chain fatty acids and other hydrophobic ligands. The encoded protein is important in the establishment of the radial glial fiber in the developing brain. Alternative splicing and promoter usage results in multiple transcript variants encoding different isoforms. Pseudogenes of this gene are found on multiple chromosomes.Immunogen information
Immunogen A synthesized peptide derived from human BLBP/FABP7. Sequence Email for sequence Gene ID Swiss Prot Synonyms MRG; BLBP; FABPB; B-FABP Calculated MW 14KDa/18KDa Observed MW 15kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:2000 - 1:6000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples U-251MG, K-562(Low expression control) Cellular location Cytoplasm 
Western blot analysis of various lysates, using BLBP/FABP7 Rabbit mAb (A23286) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse brain cells using BLBP/FABP7 Rabbit mAb (A23286) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthesized peptide derived from human BLBP/FABP7. Isotype IgG