быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PD-L2/CD273 Rabbit mAb (A23287)
- Описание
- Характеристики
-
Overview
Product name PD-L2/CD273 Rabbit mAb Catalog No. A23287 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60190 Background
Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis.Immunogen information
Immunogen Recombinant protein of human PD-L2/CD273. Sequence Email for sequence Gene ID Swiss Prot Synonyms B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2 Calculated MW 31kDa Observed MW 45-60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HUVEC, Jurkat(Low expression control) Cellular location Cell membrane, Endomembrane system, Secreted 
Western blot analysis of various lysates, using PD-L2/CD273 Rabbit mAb (A23287) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse heart using PD-L2/CD273 Rabbit mAb (A23287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat heart using PD-L2/CD273 Rabbit mAb (A23287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human PD-L2/CD273. Isotype IgG