быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SMARCA2 / BRM Rabbit mAb (A23291)
- Описание
- Характеристики
-
Overview
Product name SMARCA2 / BRM Rabbit mAb Catalog No. A23291 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59944 Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3). Sequence TLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGPSTPQKLPVPAPGGRPSPAPPAAAQPPAA Gene ID Swiss Prot Synonyms BIS; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a Calculated MW 181kDa Observed MW 220kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:2000 - 1:6000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location nucleus 
Western blot analysis of various lysates, using SMARCA2 / BRM Rabbit mAb (A23291) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human cervix cancer using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg SMARCA2 / BRM Rabbit mAb (A23291). Western blot was performed from the immunoprecipitate using SMARCA2 / BRM Rabbit mAb (A23291) at a dilition of 1:5000.
-
Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3). Isotype IgG